Fluorescent proteins from copepoda species and methods for...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A01H 1/00 (2006.01) A01K 67/00 (2006.01) C07K 14/435 (2006.01) C07K 16/18 (2006.01) C12N 15/66 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2510884

The present invention provides nucleic acid molecules encoding a fluorescent and proteins and mutants, homologues and derivatives thereof, as well as proteins and peptides encoded by these nucleic acids. The nucleic acid molecules and proteins of interest are isolated from Copepoda species. Also of interest are proteins that are substantially similar to, or derivatives, or homologues, or mutants of, the above-referenced specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies specific to the proteins and peptides of the invention. In addition, host-cells, stable cell lines and transgenic organisms comprising above-referenced nucleic acid molecules are provided. The subject protein and nucleic acid compositions find use in a variety of different applications and methods, particularly for labeling of biomolecules, cell or cell organelles. Finally, kits for use in such methods and applications are provided.

La présente invention concerne des molécules d'acides nucléiques codant une substance fluorescente, des protéines et des mutants ainsi que leurs homologues et dérivés ainsi que des protéines et des mutants codés par ces acides nucléiques. Les molécules d'acides nucléiques et les protéines d'intérêt sont isolées à partir de copépodes. L'invention concerne aussi des protéines qui sont sensiblement similaires ou sont des dérivés ou homologues ou mutants des protéines spécifiques indiquées ci-dessus. L'invention concerne aussi des fragments des acides nucléiques et les peptides qu'ils codent ainsi que des anticorps spécifiques aux protéines et aux peptides de l'invention. En outre, l'invention concerne des cellules hôtes, des lignées cellulaires stables et des organismes transgéniques comprenant les molécules d'acides nucléiques décrits dans l'invention. Les compositions de protéines et d'acides nucléiques de l'invention s'utilisent dans une multitude d'applications et de procédés, notamment pour étiqueter des biomolécules, des cellules ou des organelles de cellules. L'invention concerne finalement des kits utilisés dans ces procédés et applications.

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