Fluorescent scavenger particle immunoassay

Details Fluorescent scavenger particle immunoassay Fluorescent scavenger particle immunoassay

G - Physics

01

N

150/11, 195/145

G01N 33/52 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1125632

ABSTRACT OF THE DISCLOSURE Novel immunoassays are provided employing discrete particulate reagents for determining an analyte which is a member of a specific binding pair-ligand and homologous receptor. The assay employs as a first reagent, a member of said pair bound to an insoluble particle (particle conjugate); as a second reagent, a label which is part of a signal producing system, bound to a member of said pair (signal label conjugate); and as a third re- agent, a signal repressor comprising an inso- luble particle, where the signal repressor is obstructed from interacting with said label of said signal label conjugate, when said signal label conjugate is bound to said particle conjugate. In performing the assay, the analyte, the reagents, and any ancillary materials are combined in an aqueous assay medium and the signal determined as compared to an assay medium having a known amount of analyte. The repressor greatly enhances the sensitivity and accuracy of the immunoassay in repressing the signal produced by labels which are not bound to the particle conjugate, thus substantially limiting the observed signal to label bound to the particle conjugate. The labels which are employed provide a signal which does not differ significantly from when the signal label conjugate is bound to the particle conjugate or is free in the bulk solution. Illustrative labels include chromogens, such as fluorescers, chemiluminescers, and the like. Particular reagents and kits are provided, where the kits have predetermined amounts of the various reagents to substantially optimize the sensitivity of the assay.

341072

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