Fluorescent stokes shift probes for polynucleotide...

Details Fluorescent stokes shift probes for polynucleotide... Fluorescent stokes shift probes for polynucleotide...

C - Chemistry, Metallurgy

12

Q

150/8.5

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12Q 1/70 (2006.01) G01N 33/533 (2006.01)

Patent

CA 1273552

ABSTRACT Fluorescent stokes shift probes for polynucleotide hybridization assays are designed to provide predetermined nucleotide base unit spacings between the donor and acceptor fluorophores. When the probes are hybridized to the target polynucleotide the fluorophores paired for non-radiative energy transfer are separated by 2 to 7 nucleotide base units. The fluorophores are attached to the DNA or RNA probes by linker arms having lengths of 4 to 30 Angstroms. Fluorescein is a preferred donor for use with a Texas Red acceptor.

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