Fluorogenic reagents for detection of glycoconjugates,...

Details Fluorogenic reagents for detection of glycoconjugates,... Fluorogenic reagents for detection of glycoconjugates,...

C - Chemistry, Metallurgy

07

C

C07C 205/56 (2006.01) C07C 229/42 (2006.01) C07D 215/14 (2006.01) C07D 239/74 (2006.01) C07D 317/46 (2006.01) G01N 33/52 (2006.01) H04N 9/64 (2006.01) H04N 9/68 (2006.01)

Patent

CA 2096680

18 FLUOROGENIC REAGENTS FOR DETECTION OF GLYCOCONJUGATES, ALPHA-KETOACIDS AND DIKETONES ABSTRACT OF THE DISCLOSURE Compounds containing .alpha.-dicarbonyl groups are rendered capable of fluorescent emission by conjugation with carboxylic acid-terminated ortho-diaminoaryl reagents. The carboxylic acid groups serve as ionizable moieties which facilitate the conjugation reaction, and are retained in the conjugation product to enable, or enhance the ability of, the conjugated compounds to be separated in chromatographic and electro- migration separation processes, notably electrphoresis. Ortho-dinitro derivatives of the reagents are also disclosed, these derivatives offering the advantage of stability as well as the ability to be used directly in the reaction in the presence of a reducing agent.

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