Fluorometers

G - Physics – 01 – N

Patent

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Details

G01N 21/64 (2006.01) G01J 1/04 (2006.01) G01J 1/42 (2006.01)

Patent

CA 2550335

In apparatus for the production and detection of fluorescence at a sample surface, the height of the apparatus above the sample surface is reduced, and loss of the emitted fluorescence due to reflection loss and light scattering is minimized. The apparatus comprises a three-dimensionally curved light reflecting surface (40) that directs light from a light source (32) transversely to its original path and focuses the light on to an illumination zone (30) at or below the sample surface. The reflecting surface (40) also collects, directs and at least partially collimates emitted fluorescence transversely to its original path and towards a detector (46).

Dans un appareil de production et de détection de fluorescence au niveau de la surface d'un échantillon, la hauteur de l'appareil au-dessus de la surface d'échantillon est réduite, et la perte de la fluorescence émise en raison de la perte de réflexion et de la diffusion de la lumière, est minimisée. Cet appareil comprend une surface réfléchissant la lumière courbée et tridimensionnelle (40) qui dirige la lumière d'une source de lumière (32), dans le sens transversal, vers son trajet originel et cible la lumière sur une zone d'éclairage (30) au niveau de la surface d'échantillon ou au-dessous de celle-ci. La surface réfléchissante (40) recueille, dirige et au moins collimate également la fluorescence émise dans le sens transversal vers son trajet originel et vers un détecteur (46).

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