Fluorometric assay for detecting nucleic acid cleavage

Details Fluorometric assay for detecting nucleic acid cleavage Fluorometric assay for detecting nucleic acid cleavage

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/44 (2006.01) C12Q 1/70 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2209080

A method of detecting an enzyme-mediated DNA cleavage reaction in a fluorometric assay is provided. The method can be used to detect DNA cleavage caused by restriction endonucleases, retroviral integrase enzymes, DNases, RNases, or enzymes utilized in other strand separating processes in molecular biology.

L'invention concerne une méthode de détection fluorométrique d'une réaction de clivage d'ADN médiée par une enzyme. On peut utiliser ce procédé afin de détecter le clivage de l'ADN provoqué par des endonucléases de restriction, des enzymes d'intégrase rétrovirale, des DNases, des RNases ou des enzymes utilisées dans d'autres processus de séparation de brins en biologie moléculaire.

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