Fluorometric assay of 1gg.sub.4 and reagents therefor

Details Fluorometric assay of 1gg.sub.4 and reagents therefor Fluorometric assay of 1gg.sub.4 and reagents therefor

G - Physics

01

N

167/43

G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1259029

Abstract FLUOROMETRIC ASSAY OF IgG4 AND REAGENTS THEREFOR A method for identifying and quantifying allergen specific IgG4 levels in patient serum by conjugating the IgG4 in the serum with allergens adhering to an insoluble support, conjugating the serum IgG4 with an enzyme labeled anti-IgG4 antibody, contacting the enzyme label with a solution of a substrate which will yield a fluorescent product in the presence of the enzyme, and measuring the level of fluorescence in the solution. Special reagents and their manufacture are also disclosed.

481283

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