Fluorometric assay of total ige level and reagents therefor

Details Fluorometric assay of total ige level and reagents therefor Fluorometric assay of total ige level and reagents therefor

G - Physics

01

N

167/43, 167/44

G01N 33/543 (2006.01) G01N 33/545 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1233118

-39- Abstract of the Invention A method for identifying and quantifying total IgE levels in patient serum by conjugating the IgE in the serum with anti-IgE antibody adhering to an insoluble support, conjugating the serum IgE with an enzyme labeled anti-Ige antibody, contacting the enzyme label with a solution of a substrate which will yield a fluorescent product in the presence of the enzyme, and measuring the level of fluorescence in the solution. Special reagents and their manufacture are also disclosed.

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