Fluorometric detection using visible light

G - Physics – 01 – N

Patent

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G01N 21/64 (2006.01)

Patent

CA 2283267

Systems, devices and methods are provided for viewing a pattern of fluorophors capable of fluorescing when exposed to visible light, e.g., fluorescently stained DNA, protein or other biological material. The system includes a light source (10) emitting light in the visible spectrum, such as a fluorescent lamp used in domestic lighting, a first optical filter (30) capable of transmitting light from the source at wavelengths capable of exciting the fluorophors and of absorbing light of other wavelengths, and a second optical filter (60) capable of blocking substantially all the light from the source not blocked by the first filter, so that the only light reaching the viewer is light produced by fluorescence of the fluorophors.

L'invention concerne des systèmes, des dispositifs et des procédés permettant de visualiser une configuration de fluorophores capables de rendre fluorescents lorsqu'ils sont exposés à la lumière visible, par exemple, de l'ADN, des protéines ou une autre matière biologique, colorés avec des pigments fluorescents. Le système comprend une source de lumière (10) émettant de la lumière dans le spectre visible, telle qu'une lampe fluorescente utilisée pour l'éclairage domestique, un premier filtre optique (30) capable de transmettre la lumière provenant de la source de lumière à des longueurs d'ondes capables d'exciter les fluorophores, et d'absorber la lumière d'autres longueurs d'ondes, et un deuxième filtre optique (60) capable de bloquer sensiblement toute la lumière provenant de la source et n'ayant pas été bloquée par le premier filtre, de sorte que la seule lumière atteignant le visualiseur soit celle produite par la fluorescence des fluorophores.

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