C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2311931
A method for monitoring a reaction within a sample, said method comprising supplying or forming a double stranded polynucleotide having a fluorescence donor and a fluorescence acceptor molecule spaced along the length at a distance at which fluorescence from the donor is reduced by the acceptor, applying to said molecule a restriction enzyme which cuts said polynucleotide at a site intermediate said donor and said acceptor molecule, and monitoring fluorescence of said sample. The method is particularly suitable for detecting polynucleotide amplicification, in particular in sequence detection but may also be used in enzyme quality control.
L'invention porte sur un procédé permettant d'observer le déroulement d'une réaction dans un échantillon, consistant à fournir ou former un polynucléotide double brin comportant un donneur de fluorescence, et une molécule acceptrice de fluorescence, espacés dans le sens de la longueur d'une distance telle que la fluorescence du donneur soit réduite par la molécule acceptrice, puis à appliquer à ladite molécule acceptrice une enzyme de restriction qui coupe ledit polynucléotide en un site intermédiaire entre ledit donneur et ladite molécule acceptrice, puis enfin à observer la fluorescence dudit échantillon. Le procédé est particulièrement indiqué pour détecter l'amplification de polynucléotides, en particulier pour la détection de séquences, mais il peut également servir au contrôle de qualité des enzymes.
Lee Martin Alan
Leslie Dario Lyall
Fetherstonhaugh & Co.
The Secretary Of State For Defence In Her Britannic Majesty's Go
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