Fluorometric system, method and test article

Details Fluorometric system, method and test article Fluorometric system, method and test article

G - Physics

01

N

73/51.5

G01N 21/64 (2006.01)

Patent

CA 1086093

ABSTRACT OF THE DISCLOSURE A fluorometric system to determine the kind and amount of substances derived from a biological fluid (e.g., serum or urine) or tissue in which the substances to be detected (e.g., antigen, antibody, hormone or enzyme) are coated onto a substrate surface in fluorescent form. Multiple coating areas of different samples may be employed. The fluorometric system includes a source of filtered light to excite fluorescence, optical systems for conducting the excitation light to such coating, and optical systems for receiving emitted fluoroescence and for detecting the same. The system efficiency and optical characteristics disclosed avoid photo-bleaching; limit fading; and are especially adapted to provide accurate surface reading fluorometry.

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