Focused acoustic energy in the preparation and screening of...

B - Operations – Transporting – 01 – J

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B01J 19/00 (2006.01) B41J 2/14 (2006.01) B05B 17/06 (2006.01)

Patent

CA 2423063

The present invention provides a method for the acoustic ejection of fluid droplets (49) from each of a plurality of fluid-containing reservoirs (13, 15) to prepare combinatorial libraries in the form of microarrays. An acoustic ejection device (11) is used comprised of a plurality of fluid reservoirs (13, 15), an ejector (33) for generating acoustic radiation and focusing the acoustic radiation generated at a focal point sufficiently near the fluid surface (17, 19) in each of the reservoirs (13, 15) such that a fluid droplet (49) is ejected therefrom toward a site on a substrate surface (51), and a means for positioning (43) the ejector (33) in acoustically coupled relationship to each of the reservoirs (13, 15). The combinatorial libraries may comprise biological or nonbiological moieties.

La présente invention concerne une méthode d'éjection acoustique de gouttelettes liquides de plusieurs réservoirs contenant un liquide afin de préparer des bibliothèques combinatoires sous forme de micro-réseaux. Le dispositif d'éjection acoustique utilisé comprend plusieurs réservoirs de liquide, un éjecteur servant à générer un rayonnement acoustique et à focaliser le rayonnement acoustique généré sur un point de focalisation suffisamment près de la surface du liquide dans chacun des réservoirs, de manière qu'une gouttelette de liquide en soit éjectée vers un site sur la surface d'un substrat, et une unité de positionnement de l'éjecteur dans une relation de couplage acoustique à chacun des réservoirs. Les bibliothèques combinatoires peuvent comprendre des fragments biologiques ou non biologiques.

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