C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/74 (2006.01) A23C 9/12 (2006.01) C12N 1/21 (2006.01) C12N 9/24 (2006.01) C12N 9/40 (2006.01) C12N 9/52 (2006.01) C12N 15/55 (2006.01) C12N 15/63 (2006.01)
Patent
CA 2056056
ABSTRACT A food-grade vector is provided which is suitable for transforming afood-grade host cell and is incapable of replicating autonomously in said host cell, said vector comprising: 1) at least one stretch of nucleotides capable of hybridizing with chromosomal DNA of the non-transformed host cell enabling said vector to integrate stably into the chromosome of said host cell after transformation and 2) at least one stretch of foreign DNA selected from: a) stretches comprising at least one DNA sequence that codes for at least one product enabling the transformed host cell to metabolize a substrate that cannot be metabolized by the non- transformed host cell, wherein at least said DNA sequence and the product encoded by said DNA sequence are foreign for a prototrophic strain of said non-transformed host cell b) stretches comprising at least one DNA sequence that codes for at least one product enabling the transformed host cell to grow in the presence of a food grade, natural bacteriocidal agent, wherein at least said DNA sequence and the product encoded by said sequence are foreign for the non-transformed host cell. The food-grade host is preferably a lactic acid bacterium. The foreign DNA preferably codes for an enzyme essential for metabolizing an oligo- saccharide or codes for a proteinase and a maturase or codes for a food- grade natural bacteriocidal agent and/or immune proteins for such an agent and/or proteins involved in the proper secretion of such an agent.
Leenhouts Cornelis J.
Marugg John D.
Verrips Cornelis T.
Leenhouts Cornelis J.
Marugg John D.
Plc Unilever
Ridout & Maybee Llp
Verrips Cornelis T.
LandOfFree
Food-grade vector suitable for transforming a food-grade... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Food-grade vector suitable for transforming a food-grade..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Food-grade vector suitable for transforming a food-grade... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-2009518