Formation of colloidal metal dispersions using aminodextrans...

C - Chemistry – Metallurgy – 07 – K

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C07K 17/14 (2006.01) A61K 47/48 (2006.01) B01J 13/00 (2006.01) B22F 9/24 (2006.01) C12N 11/14 (2006.01) G01N 33/531 (2006.01) G01N 33/548 (2006.01) G01N 33/553 (2006.01)

Patent

CA 2128230

The invention related generally to the preparation of colloidal metal(O) particles having a crosslinked aminodextran coating with pendent amine groups attached thereto. The aminodextran acts as both a reductant for reducing metal ions to metal(O) particles and as the protective agent which coats the metal(O) particles thus formed. After stabilizing the aminodextran coating by use of a crosslinking agent, the coated particles can be used to covalently bind proteins. The resulting protein containing colloidal particles can be used as markers in optical and electron microscopy, in immunological and biological assays, and possibly as therapeutic agents.

Préparation de particules de métal(O) colloïdales possédant un revêtement d'aminodextrane réticulé auquel sont liés des groupes amine dépendants. L'aminodextrane agit aussi bien comme réducteur pour réduire les ions métal en particules de métal(O) que comme agent protecteur qui recouvre les particules de métal(O) ainsi formées. Après stabilisation du revêtement d'aminodextrane à l'aide d'un agent réticulant, les particules revêtues peuvent être utilisées pour lier des protéines de manière covalente. Les particules colloïdales contenant des protéines résultantes peuvent être utilisées comme marqueurs en microscopie optique et électronique, dans des essais immunologiques et biologiques, et éventuellement comme agents thérapeutiques.

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