Formation of immobilized biological layers for sensing

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/00 (2006.01) C12N 11/00 (2006.01) G01N 27/30 (2006.01) G01N 27/327 (2006.01) G01N 27/333 (2006.01) G01N 27/416 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) C12M 1/40 (2006.01) G01N 21/77 (2006.01)

Patent

CA 2710498

The invention is di-rected to enzyme immobilization compositions comprising: one or more enzymes, a humectant, an acrylic-based monomer, a water-solu-ble organic photo-initiator and a wa-ter-soluble acrylic-based cross-linker in a substantially homogeneous aque-ous mixture. The invention is also di-rected to methods for forming sensors comprising such compositions and to apparati for forming arrays of immo-bilized layers on an array of sensors by dispensing such compositions onto a substrate.

L'invention porte sur des compositions d'immobilisation d'enzymes comprenant : une ou plusieurs enzymes, un humectant, un monomère à base acrylique, un photoinitiateur organique soluble dans l'eau et un réticulant à base acrylique soluble dans l'eau dans un mélange aqueux sensiblement homogène. L'invention porte également sur des procédés de formation de détecteurs comprenant de telles compositions et sur un appareil pour former des réseaux de couches immobilisées sur un réseau de détecteurs par distribution de telles compositions sur un substrat.

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