Formation of layers of amphiphilic molecules

G - Physics – 01 – N

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G01N 33/487 (2006.01) B01D 67/00 (2006.01)

Patent

CA 2708624

To form a layer (11) separating two volumes of aqueous solution, especially a biolayer lipid membrane (BLM) there is used an apparatus comprising elements defining a chamber (7), the elements including a body (2) of non-conductive material having formed therein at least one recess (5) opening into the chamber, the recess containing an electrode (21). A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.

Pour former une couche séparant deux volumes de solution aqueuse, on utilise un appareil comprenant des éléments délimitant une chambre, les éléments comprenant un corps en matière non conductrice ayant, formée dans celui-ci, au moins une cavité s'ouvrant dans la chambre, la cavité contenant une électrode. Un revêtement de prétraitement constitué d'un fluide hydrophobe est appliqué sur le corps dans toute la cavité. On fait couler une solution aqueuse, ayant des molécules amphiphiles ajoutées à celle-ci, d'un côté à l'autre du corps pour recouvrir la cavité de sorte que la solution aqueuse est introduite dans la cavité à partir de la chambre et qu'une couche des molécules amphiphiles se forme dans toute la cavité séparant un volume de solution aqueuse introduit dans la cavité du volume restant de solution aqueuse.

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