Formation of thermostable enzymes with extra-ordinary heat...

C - Chemistry – Metallurgy – 07 – K

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C07K 17/06 (2006.01) C07F 7/18 (2006.01) C07F 9/10 (2006.01) C12N 11/06 (2006.01) C12N 11/14 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2064683

ABSTRACT Enzymes and certain other bioactive substances are immobilized on solid substrates which have sufficient functional groups such as hydroxyl or carboxyl. The bioactive substances are linked to the substrates through spacer compounds having a long open alkyl chain with 7-18 carbon atoms and optionally also through phospholipid intermediates. The spacer compound is chemically linked to the substrate. The phospholipid, where used, is covalently linked to the spacer compound. Methods of making the immobilized preparations as well as the intermediate compounds are described. The preparations of the invention exhibit a marked increase in activity and stability of the immobilized biosubstances tested over prior art preparations.

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