Forming membrane structure by applying protein suspension to...

B - Operations – Transporting – 01 – D

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18/32, 117/88.5

B01D 69/02 (2006.01) B01D 67/00 (2006.01) B01D 69/14 (2006.01) B01D 71/74 (2006.01) C12N 1/06 (2006.01) C12N 11/04 (2006.01)

Patent

CA 1307632

ABSTRACT Disclosed is a method for producing a structure such as ultra filtration membrane. The structure comprises a membrane which has continuous pores and a plain, curved, cylindrical or vesicular surface consisting essentially of a cell wall layer of contiguous protein molecules or protein containing molecules arranged to form a crystal lattice defining the continuous pores. The membrane is connected to or embedded in a carrier or the membrane is a stable unsupported film. The method comprises removing cell contents from intact cells of microorganisms or breaking the intact cells and separating the cell wall layers or their fragments, suspending the cell wall layers or their fragments in a liquid medium, depositing or introducing the cell wall layers or their fragments onto a carrier or connecting the cell wall layers or their fragments together to form a stable unsupported film and treating with a mono- or bifunctional foreign molecule to covalently cross link the cell wall layers or their fragments inter- or intramolecularly or with the carrier. The method does not employ a disintegration of a surface layer of cell wall into its constituting protein molecules and a self-assembly of the molecules. The structure is useful, for example, for filtering or enclosing enzymes.

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