C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 7/02 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2687119
The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool.
La présente invention concerne un procédé de purification d'un virus ou d'un antigène viral consistant à prendre une préparation virale et à centrifuger cette préparation virale dans un gradient de sucre obtenu par addition de plusieurs couches de sucre tamponné de concentration différente. Ce procédé donne des rendements plus élevés et réduit l'agrégation non voulue du virus et de l'antigène viral par augmentation du volume du groupe de crête.
Grillberger Leopold
Mitterer Artur
Mundt Wolfgang
Reiter Manfred
Schafhauser Horst
Baxter Healthcare S.a.
Baxter International Inc.
Sim & Mcburney
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