Freeze-tolerant eukaryotic cells

Details Freeze-tolerant eukaryotic cells Freeze-tolerant eukaryotic cells

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A21D 6/00 (2006.01) A21D 8/04 (2006.01) C07K 14/395 (2006.01) C07K 14/705 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2446022

The present invention relates to the use of proteins facilitating water diffusion or water transport through the cell membrane, preferably aquaporin or aquaporin related proteins to obtain freeze-tolerant eukaryotic cells, preferably yeast cells or plant cells. It relates further to a method for obtaining such cells, and to freeze-tolerant cells, characterized by an enhanced expression level of proteins facilitating water diffusion or water transport through the cell membrane.

L'invention concerne l'utilisation de prot~ines facilitant la diffusion ou le transport d'eau ~ travers la membrane cellulaire, de pr~f~rence, l'aquaporine ou des prot~ines relatives ~ l'aquaporine, de mani­re ~ obtenir des cellules eucaryotes tol~rant le gel, de pr~f~rence des cellules de levure ou des cellules v~g~tales. L'invention concerne ~galement un proc~d~ permettant d'obtenir de telles cellules et des cellules tol~rant le gel, caract~ris~es par un niveau accru d'expression de prot~ines facilitant la diffusion ou le transport d'eau ~ travers la membrane cellulaire.

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