Fret probes and methods for detecting interacting molecules

Details Fret probes and methods for detecting interacting molecules Fret probes and methods for detecting interacting molecules

G - Physics

01

N

G01N 33/58 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2505515

This invention relates to the field of detecting interacting molecules. The invention provides a set of a first and a second probe each probe provided with a dye allowing energy transfer at least one probe provided with a reactive group allowing juxtaposing said first and second probe. A method is provided for detecting at least two interacting molecules at the single cell level using of a set of probes according to the invention.

L'invention concerne le domaine de la détection de molécules d'interaction. Cette invention fournit un ensemble constitué d'une première et d'une seconde sonde. Chaque sonde est équipée d'un colorant permettant le transfert d'énergie, au moins une de celles-ci étant équipée d'un groupe réactif permettant la juxtaposition desdites sondes. L'invention concerne également un procédé de détection d'au moins deux molécules d'interaction au niveau d'une cellule unique au moyen de l'ensemble de sondes de la présente invention.

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