Fructose polymer synthesis in monocot plastids

Details Fructose polymer synthesis in monocot plastids Fructose polymer synthesis in monocot plastids

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A23L 1/0522 (2006.01) C07H 3/06 (2006.01) C12N 9/10 (2006.01) C12P 19/04 (2006.01)

Patent

CA 2386629

This invention concerns methods for synthesis and accumulation of fructo- oligosaccharides in seeds of monocot plants by selective expression of a fructosyltransferase gene sequence. Selective expression includes coordination of timing, tissue specific expression and especially subcellular location. Successful transformants utilize sucrose to synthesize and accumulate fructo- oligosaccharides in the plastid of the cell, in established crops, without loss of co-products or concern for yield loss due to degradation during maturation, harvest or storage of the plant. Enhanced fructo-oligosaccharide production will benefit the fructose sweetener industry and add value to grain used for feed.

Cette invention concerne des techniques de synthèse et l'accumulation de fructo-ologosaccharides dans des graines de plantes monocolylédones grâce à l'expression sélective d'une séquence génique de fructosyltransférase. Cette expression sélective concerne la coordination dans le temps, l'expression spécifique de tissus et la localisation subcellulaire. Les transformants qui donnent de bons résultats font intervenir la sucrose pour la synthèse et l'accumulation de fructo-oligosaccharides dans le plaste de la cellule, dans des cultures établies, sans perte de co-produits importants pour le rendement par suite de la dégradation survenue pendant la maturation, la récolte ou le stockage de la plante. La production accrue de fructo-oligo-saccharides sera bénéfique pour l'industrie des édulcorants à base de fructose et valorisera les grains utilisés dans l'alimentation animale.

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