Fructosyltransferases (inulosucrase and levansucrase) from...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) A23C 9/123 (2006.01) A61K 35/74 (2006.01) C08B 37/00 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12P 19/18 (2006.01)

Patent

CA 2409965

The present invention describes two novel proteins having fructosyltransferase activity. Both enzymes are derived from lactobacilli, which are food-grade microorganisms with the Generally Recognized As Safe (GRAS) status. One of these proteins produces an inulin and fructo-oligosaccharides, while the other produces a levan. According to the invention lactobacilli capable of producing an inulin and/or a levan and/or fructo-oligosaccharides using one or both of the fructosyltransferases can be used as a probiotic or a symbiotic.

L'invention concerne deux nouvelles protéines possédant une activité fructosyltransférase. Ces deux enzymes sont dérivées des lactobacilles, qui sont des micro-organismes de classe alimentaire dotés du statut GRAS (Généralement Reconnu Comme Fiable). L'une des ces protéines produit une inuline et des fructo-oligosaccharides, alors que l'autre produit un lévane. Selon la présente invention, les lactobacilles capables de produire une inuline et/ou un lévane et/ou des fructo-oligosaccharides au moyen d'un ou des deux fructosyltransférase(s) peuvent servir de probiotique ou de symbiotique.

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