Full term development of animals from enucleated oocytes...

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2318070

Animals are produced following injection of adult somatic cell nuclei into enucleated oocytes. The invention provides a method for cloning an animal by directly inserting at least a portion of an adult somatic cell nucleus into a recipient enucleated oocyte. Preferably, the nucleus is inserted by microinjection and, more preferably, by piezo electrically-actuated microinjection. The oocyte is activated prior to, during, or up to about 6 hours after insertion of the nucleus, by electroactivation or exposure to a chemical activating agent, such as Sr2+. The activated renucleated oocyte is allowed to develop into an embryo and is transplanted to a host surrogate mother to develop into a live offspring.

Des animaux ont été produits suite à l'injection de noyaux de cellules somatiques adultes dans des ovocytes énucléés. On décrit dans cette invention un procédé permettant de cloner un animal qui consiste à insérer directement au moins une partie d'un noyau de cellule somatique adulte dans un ovocyte énucléé récepteur. Le noyau est de préférence introduit par micro-injection et, plus préférablement encore par micro-injection à commande piézo-électrique. L'ovocyte est activé avant, pendant et jusqu'à 6 heures avant l'introduction du noyau, par électro-activation ou exposition à un agent d'activation chimique, tel que Sr?2+¿. On laisse l'ovocyte rénucléé activé se développer sous forme d'embryon puis on le transplante chez une mère porteuse réceptrice pour qu'il se développe et devienne un bébé animal vivant.

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