Function homology screening

Details Function homology screening Function homology screening

G - Physics

01

N

G01N 33/53 (2006.01) G01N 33/50 (2006.01) G01N 33/567 (2006.01) G01N 33/68 (2006.01) G01N 5/00 (2006.01)

Patent

CA 2400989

A method of screening biologically active agent based on the analysis of complex biological responses in culture. Methods for selecting cells and culture conditions for such screens are provided, as well as the identification of an optimized set of discrete parameters to be measured, and the use of biomap analysis for rapid identification and characterization of drug candidates, genetic sequences acting pathways, and the like. A feature of the invention is simultaneous screening of a large number of cellular pathways, and the rapid identification of compounds that cause cellular responses.

L'invention a trait à une méthode de criblage d'un agent biologiquement actif basée sur l'analyse de réponses biologiques complexes en culture. L'invention concerne également des méthodes de sélection de cellules et de conditions de culture pour ces cribles, de même que l'identification d'un ensemble optimisé de paramètres distincts à mesurer, et l'utilisation d'une analyse d'une biocarte permettant l'identification et la caractérisation rapides de candidats médicaments, de voies agissant sur les séquences génétiques, et analogue. Une caractéristique de l'invention est le criblage simultané d'un grand nombre de voies cellulaires, ainsi que l'identification rapide de composés provoquant des réponses cellulaires.

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