C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2144768
A method for determining whether an individual, i.e., a human, carries a mutation which encodes a polypeptide which is a transcriptional regulator including the steps of obtaining a first nucleic acid comprising cDNA encoding both alleles of the gene from a cell sample from the individual, providing a second nucleic acid comprising a binding site operably linked to DNA encod- ing a detectable protein such that transcription of the DNA encoding the detectable protein is regulated by interaction of the tu- mor suppressor polypeptide with the binding site, transfecting these first and second nucleic acids into cells with low levels of the tumor suppressor polypeptide, and then examining the transfected cells for the level of the detectable protein, the level being correlated with the presence of a mutation in at least one of the alleles of the gene.
Frebourg Thierry
Friend Stephen H.
Iggo Richard
Ishioka Chikashi
Institut Suisse de Recherches Experimentales Sur Le Cancer
Smart & Biggar
The General Hospital Corporation
LandOfFree
Functional assay for tumor supressor genes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Functional assay for tumor supressor genes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Functional assay for tumor supressor genes will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1760069