Functional protein arrays

Details Functional protein arrays Functional protein arrays

C - Chemistry, Metallurgy

40

B

C40B 50/06 (2006.01) C07K 1/04 (2006.01) C07K 17/00 (2006.01) C07K 17/06 (2006.01) C12N 15/00 (2006.01) C12P 21/02 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01) C12Q 1/68 (2006.01) C40B 40/10 (2006.01) C40B 50/14 (2006.01)

Patent

CA 2419490

The present invention relates to a method for producing a protein array starting from DNA (or mRNA) in which a number of native, functional proteins, domains or peptides are produced in parallel by in in vitro synthesis using a cell free system for trancription and translation. The products are immobilised in a gridded format on a surface, using an isolation sequence tag incorporated into the proteins.

L'invention concerne un procédé de préparation d'un réseau de protéines à partir d'un ADN (ou ARNm) dans lequel un certain nombre de protéines natives et fonctionnelles, de domaines ou de peptides sont produits en parallèle par synthèse in vitro, au moyen d'un système exempt de cellule aux fins de transcription et de traduction. Les produits sont immobilisés, sous forme de grille sur une surface, au moyen d'une étiquette de séquence d'isolation incorporée dans les protéines.

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