Functional screening method

Details Functional screening method Functional screening method

G - Physics

01

N

G01N 33/50 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2493409

The present invention provides high-throughput functional genomic methods for determininggene and protein function in a cellular context. Also provided are methods for identifying chemical modulators of gene and protein/enzyme activity. Assays are generated in concert with screening in an iterative process which expands the scope of biological coverage with each iteration and which uses image-based analysis to yield data at sub-cellular resolution.

L'invention concerne des procédés génomiques fonctionnels à rendement élevé permettant de déterminer une fonction génique et protéique dans un contexte cellulaire. L'invention concerne également des procédés d'identification de modulateurs chimiques d'une activité génique et protéique/enzymatique. Des tests sont produits en accord avec le criblage dans un processus itératif élargissant l'ampleur de la couverture biologique avec chaque itération et utilisant une analyse fondée sur l'image de manière à produire des données à une résolution sous-cellulaire.

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