Functionalization of t cell derived vesicles and use thereof...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 5/0783 (2010.01) A61K 39/00 (2006.01) A61K 39/12 (2006.01) A61K 39/29 (2006.01) C07K 14/18 (2006.01) C07K 17/02 (2006.01)

Patent

CA 2479021

The present invention relates to compositions comprising vesicles released' from activated T lymphocytes, as well as to methods for their production and uses. Said vesicles contain a set of bioactive molecules which confer remarkable properties, such as antigen recognition, antigen presentation and other regulatory and effector functions. This invention also relates to methods for transferring or delivering antigenic molecules (e.g., peptides, peptide/MHC complexes, TCR or subunit thereof, etc.) to antigen presenting cells (APCs) using said vesicles, to induce specific immune responses, particularly specific CTL responses. The invention further relates to methods of delivering molecules selectively or specifically to target cells using said vesicles.

L'invention concerne des compositions contenant des vésicules libérées par les lymphocytes T activés, et des procédés de production et d'utilisation de celles-ci. Ces vésicules contiennent un ensemble de molécules bioactives qui leur confèrent des propriétés remarquables telles que reconnaissance d'antigène, présentation d'antigène et d'autres fonctions régulatrices et effectrices. L'invention concerne aussi des procédés permettant de transférer ou d'apporter des molécules antigéniques (p. ex. peptides, complexes peptide/CMH, TCR ou sous-unité de celui-ci, etc.) à des cellules présentatrices d'antigène (APC) au moyen desdites vésicules, afin d'induire des réactions immunitaires spécifiques, en particulier des réactions de cellules T cytotoxiques spécifiques. L'invention concerne de plus des procédés d'apport sélectif ou spécifique de molécules à des cellules cibles au moyen de ces vésicules.

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