C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/00 (2006.01) G06F 19/00 (2006.01)
Patent
CA 2534710
The inventors have developed a method of altering the amino acid sequence of a fun-gal alpha-amylase to obtain variants, and they have used the method to construct such vari-ants. The variants may be useful for anti-staling in baked products. Accordingly, the invention provides a method of constructing fungal alpha-amylase variants based on a comparison of three-dimensional (3D) structures of the fungal alpha-amylase and a maltogenic alpha-amylase. One or both models includes a substrate. The in-vention also provides novel fungal alpha-amylase variants.
La présente invention se rapporte à un procédé de modification de la séquence d'acides aminés d'une alpha-amylase fongique aux fins de l'obtention de variants, ainsi qu'à la mise en oeuvre de ce procédé pour élaborer de tels variants. Ces variants peuvent être utilisés dans des produits cuits au four pour empêcher leur rassissement. En conséquence, l'invention se rapporte à un procédé d'élaboration de variants d'alpha-amylases fongiques fondé sur une comparaison des structures tridimensionnelles (3D) de l'alpha-amylase fongique et d'une alpha-amylase maltogénique. L'un ou chacun des deux modèles comprend un substrat. L'invention se rapporte également à de nouveaux variants d'alpha-amylases fongiques.
Beier Lars
Jensen Morten Tovborg
Spendler Tina
Svendsen Allan
Vind Jesper
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Novozymes A/s
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