Fungal peroxygenases and methods of application

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/08 (2006.01)

Patent

CA 2682451

The invention relates to polypeptides having peroxygenase activity and compositions comprising such polypeptides, their encoding polynucleotides, expression vectors and recombinant host cells comprising such polynucleotides or vectors, methods of producing the polypeptides, as well as methods of application and uses thereof, including a process for enzymatic, regioselective oxygenation of N-heterocycles of the general formula (I) to the corresponding N-oxides of the formula (II), by converting N-heterocycles of the formula (I) with a peroxidase polypeptide in the presence of at least one oxidizing agent in a one-stage reaction process.

L'invention porte sur des polypeptides à activité peroxygénase et des compositions renfermant lesdits polypeptides, sur leurs polynucléotides codants, sur des vecteurs d'expression et des cellules hôtes recombinantes comprenant lesdits polynucléotides ou vecteurs, sur des procédés de production des polypeptides, ainsi que sur des procédé d'application et des utilisations de ces derniers, y compris un procédé qui permet une oxygénation enzymatique régiosélective de N-hétérocycles de la formule générale (I) en N-oxydes correspondants, les N-hétérocycles de la formule (I) étant convertis au moyen d'un polypeptide peroxydase en présence d'au moins un agent oxydant au cours d'un processus de réaction en une étape.

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