Fungal promoters active in the presence of glucose

Details Fungal promoters active in the presence of glucose Fungal promoters active in the presence of glucose

C - Chemistry, Metallurgy

12

N

C12N 15/80 (2006.01) C07K 14/37 (2006.01) C12N 9/42 (2006.01) C12N 15/11 (2006.01) C12N 15/56 (2006.01)

Patent

CA 2142602

2142602 9404673 PCTABS00030 A method is described for the identification and cloning of promoters that express under a defined environmental condition, such as growth in glucose medium. Using this method, five Trichodermal promoters capable of the high expression of operably linked coding sequences are identified, one of which is the promoter for T. reesei tef1. Also provided are altered cbh1 promoters, altered so that glucose no longer represses expression from such promoter. The invention further provides vectors and hosts that utilize such promoters, and unique fungal enzyme compositions from such hosts.

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