Fused liposome and acid induced method for liposome fusion

Details Fused liposome and acid induced method for liposome fusion Fused liposome and acid induced method for liposome fusion

A - Human Necessities

61

K

167/163, 117/46.

A61K 9/50 (2006.01) A61K 9/127 (2006.01) A61K 9/42 (2006.01) A61K 39/00 (2006.01) A61K 39/02 (2006.01) C12N 15/88 (2006.01)

Patent

CA 1261751

Abstract of the Disclosure Liposomes containing phosphatidylethanolamine, palmitoyl homocysteine or oleic acid or palmitic acid, fuse rapidly when the pH of the medium is reduced below 7. Liposome fusion was measured by (a) mixing of the liposomal lipids as shown by resonance energy transfer, (b) gel filtration and (c) electron microscopy. The presence of phosphatidylethanolamine or acid addition esters thereof in the liposomes greatly enhances fusion; whereas the presence of phosphatidylcholine inhibites fusion. During fusion of liposomes oontaining phosphatidylethanolamine:palmitoyl homocysteine (8:2), almost all of the encapsulated calcein is released. Inclusion of cholesterol (40%) in the liposomes substantially decreases leakage without impairing fusion. Those pH sensitive liposomes are fused to deliver biologically active molecules such as DNA, into living cells.

479526

Affiliated with

Also associated with

Rating

Fused liposome and acid induced method for liposome fusion does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fused liposome and acid induced method for liposome fusion, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fused liposome and acid induced method for liposome fusion will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1336360