C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
530/13, 167/130,
C12N 15/62 (2006.01) A61K 39/245 (2006.01) C07K 14/035 (2006.01) C07K 14/245 (2006.01) C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/38 (2006.01) C12P 21/02 (2006.01) A61K 39/00 (2006.01)
Patent
CA 2024918
TITLE: FUSED PROTEINS AND PRODUCTION THERFEOF ABSTRACT Disclosed are (1) a fused protein comprising heat-labile enterotoxin B subunit and a protein heterologous to heat-labile enterotoxin, (2) a recombinant DNA containing a nucleotide sequence coding for the above fused protein, (3) a transformant harboring the above recombinant DNA, (4) a method for producing the fused protein which comprises cultivating the above transformant, producing and accumulating the above fused protein in a culture, and collecting the fused protein, and (5) a method for purifying a fused protein comprising a herpes simplex virus surface antigen and heat-labile enterotoxin B subunit, which comprises cultivating a transformant harboring a recombinant DNA containing a nucleotide sequence coding for the fused protein, producing an accumulating the fused protein in a culture, collecting the fused protein and subjecting the collected fused protein to purification processes comprising cationic exchange chromatography and gel permeation chromatography.
Fujisawa Yukio
Hinuma Shuji
Mayumi Aki
Yamamoto Tatsuo
Fetherstonhaugh & Co.
Takeda Chemical Industries Ltd.
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