Fused proteins and production thereof

Details Fused proteins and production thereof Fused proteins and production thereof

C - Chemistry, Metallurgy

12

N

530/13, 167/130,

C12N 15/62 (2006.01) A61K 39/245 (2006.01) C07K 14/035 (2006.01) C07K 14/245 (2006.01) C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/38 (2006.01) C12P 21/02 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2024918

TITLE: FUSED PROTEINS AND PRODUCTION THERFEOF ABSTRACT Disclosed are (1) a fused protein comprising heat-labile enterotoxin B subunit and a protein heterologous to heat-labile enterotoxin, (2) a recombinant DNA containing a nucleotide sequence coding for the above fused protein, (3) a transformant harboring the above recombinant DNA, (4) a method for producing the fused protein which comprises cultivating the above transformant, producing and accumulating the above fused protein in a culture, and collecting the fused protein, and (5) a method for purifying a fused protein comprising a herpes simplex virus surface antigen and heat-labile enterotoxin B subunit, which comprises cultivating a transformant harboring a recombinant DNA containing a nucleotide sequence coding for the fused protein, producing an accumulating the fused protein in a culture, collecting the fused protein and subjecting the collected fused protein to purification processes comprising cationic exchange chromatography and gel permeation chromatography.

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