Fusion collagenase in which affinity tag is linked and...

Details Fusion collagenase in which affinity tag is linked and... Fusion collagenase in which affinity tag is linked and...

C - Chemistry, Metallurgy

12

N

C12N 15/57 (2006.01) C12N 9/50 (2006.01) C12N 9/52 (2006.01) C12N 15/00 (2006.01) C07K 1/22 (2006.01)

Patent

CA 2744286

A fusion collagenase in which an affinity tag is added to the carboxyl terminal of a collagenase was expressed as a recombinant protein. It was found that a collagenase having a collagen-binding domain can be selectively collected by purifying the obtained fusion collagenase by affinity chromatography.

Selon linvention, une collagénase de fusion obtenue par la fixation d'un marqueur d'affinité à une extrémité terminale carboxyle d'une collagénase, est exprimée en tant que protéine recombinante. Par la purification de la collagénase de fusion ainsi obtenue par une chromatographie d'affinité, une collagénase ayant un site de liaison au collagène peut être prélevée de façon sélective.

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