Fusion proteins comprising a non-cytotoxic protease, a...

Details Fusion proteins comprising a non-cytotoxic protease, a... Fusion proteins comprising a non-cytotoxic protease, a...

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) A61K 38/16 (2006.01) A61K 38/48 (2006.01) C07K 14/33 (2006.01) C07K 14/47 (2006.01) C07K 14/635 (2006.01) C07K 14/705 (2006.01) C12N 15/12 (2006.01) C12N 15/16 (2006.01) C12N 15/55 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2593707

The invention provides a single chain, polypeptide fusion protein, comprising: a non-cytotoxic protease, or a fragment thereof, which protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of a target cell; a Targeting Moiety that is capable of binding to a Binding Site on the target cell, which Binding Site is capable of undergoing endocytosis to be incorporated into an endosome within the target cell; a protease cleavage site at which site the fusion protein is cleavable by a protease, wherein the protease cleavage site is located between the non-cytotoxic protease or fragment thereof and the Targeting Moiety; and a translocation domain that is capable of translocating the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the target cell.

L'invention concerne une chaîne simple de protéines de fusion polypeptidiques, comprenant : une protéase non-cytotoxique, ou un fragment de celle-ci, ladite protéase ou le fragment de protéase étant capable de cliver une protéine de l'appareil de fusion exocytique d'une cellule cible ; une fraction de ciblage qui est capable de liaison à un site de liaison sur la cellule cible, ledit site de liaison étant capable de subir une endocytose pour être incorporé dans une endosome dans la cellule cible ; un site de clivage de protéase, site sur lequel la protéine de fusion est clivable par une protéase, le site de clivage de la protéase étant situé entre la protéase non-cytotoxique ou un fragment de celle-ci, et la fraction de ciblage ; et un domaine de translocation qui est capable d'effectuer la translocation de la protéase ou d'un fragment de protéase, à partir de l'intérieur d'une endosome, à travers la membrane endosomique, et dans le cytosol de la cellule cible.

Affiliated with

Also associated with

Rating

Fusion proteins comprising a non-cytotoxic protease, a... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fusion proteins comprising a non-cytotoxic protease, a..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fusion proteins comprising a non-cytotoxic protease, a... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1857831