Fusion proteins containing a hinge region for enhanced cleavage

Details Fusion proteins containing a hinge region for enhanced cleavage Fusion proteins containing a hinge region for enhanced cleavage

C - Chemistry, Metallurgy

12

N

530/15.12, 195/1

C12N 15/62 (2006.01) C07K 14/485 (2006.01) C07K 14/585 (2006.01) C07K 19/00 (2006.01) C12N 15/66 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1339208

Disclosed is a method for the isolation and purification of polypeptides expressed in host cells by recombinant DNA techniques. A fused polypeptide is produced containing a desired polypeptide fused to additional amino acids. The additional amino acids define a leader sequence having properties exploitable in purification, a hinge region, and a cleavage site. The hinge region is cysteine-free and has a secondary structure which serves to expose the cleavage site to a selected endopeptidase. The method of the invention involves the production of a fused polypeptide which may be efficiently isolated by exploiting the properties of the leader sequence, and then efficiently cleaved at the cleavage site in an appropriate aqueous environment by virtue of the influence of the hinge on the cleavage agent/cleavage site reaction and other properties of the fused polypeptide.

561913

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