Fusion proteins for use in enzymatic synthesis of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) C12N 9/00 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12N 9/90 (2006.01) C12N 15/52 (2006.01) C12N 15/54 (2006.01) C12N 15/61 (2006.01) C12P 19/26 (2006.01)

Patent

CA 2315010

This invention provides fusion polypeptides that include a glycosyltransferase catalytic domain and a catalytic domain from an accessory enzyme that is involved in making a substrate for a glycosyltransferase reaction. Nucleic acids that encode the fusion polypeptides are also provided, as are host cells for expressing the fusion polypeptides of the invention.

L'invention concerne des polypeptides hybrides qui renferment un domaine catalytique d'une glycosyltransférase et un domaine catalytique d'une enzyme accessoire intervenant pendant la production d'un substrat destiné à réagir dans un cycle de glycosyltransférase. Font aussi l'objet de cette invention des acides nucléiques codant les polypeptides hybrides servant de cellules hôtes exprimant lesdits polypetides hybrides.

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