Fusion proteins having an in vivo post-translational...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) C07K 1/22 (2006.01) C07K 14/245 (2006.01) C07K 14/415 (2006.01) C07K 19/00 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 9/88 (2006.01) C12N 15/52 (2006.01) C12N 15/64 (2006.01) C12N 15/70 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2057908

2057908 9014431 PCTABS00002 A hybrid DNA sequence encoding a fusion protein comprising: a first DNA sequence encoding an amino acid sequence allowing for post-translational modification and operatively linked to a second DNA sequence encoding a selected protein or polypeptide. Included in this invention would be a vector containing this sequence, a host containing this vector, and the host in a system allowing for the production of this fusion protein. Also included is a fusion protein equivalent to the protein encoded for by this DNA sequence, in addition a method of isolating the fusion protein by bringing it into contact with a binding partner which binds the protein only after it has been modified, separating the fusion protein/binding partner complex from unbound material, and eluting the fusion protein from its binding partner would be included.

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