Fusion proteins, their preparation and use

Details Fusion proteins, their preparation and use Fusion proteins, their preparation and use

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/62 (2006.01)

Patent

CA 2065146

Fusion proteins are obtained in high yields if a mixed oligonucleotide is constructed which codes for the ballast constituent of the fusion protein. The oligonucleotide mixture is introduced in a vector in such a manner that it is functionally linked to a re- gulatory region and to the structural gene for the desired protein. Appropriate host cells are transformed with the plasmid popu- lation obtained in this manner and the clones producing a high yield of coded fusion protein are selected.

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