Fusions of scaffold proteins with random peptide libraries

Details Fusions of scaffold proteins with random peptide libraries Fusions of scaffold proteins with random peptide libraries

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07K 14/435 (2006.01) C12N 7/00 (2006.01) C12N 15/12 (2006.01) C12N 15/62 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2345215

The invention relates to the use of scaffold proteins, particularly green fluorescent protein (GFP), in fusion constructs with random and defined peptides and peptide libraries, to increase the cellular expression levels, decrease the cellular catabolism, increase the conformational stability relative to linear peptides, and to increase the steady state concentrations of the random peptides and random peptide library members expressed in cells for the purpose of detecting the presence of the peptides and screening random peptide libraries. N-terminal, C-terminal, dual N- and C- terminal and one or more internal fusions are all contemplated. Novel fusions utilizing self-binding peptides to create a conformationally stabilized fusion domain are also contemplated.

L'invention concerne l'utilisation de protéines d'échafaudage, notamment de protéines à fluorescence verte, dans des constructions de fusion avec des peptides aléatoires et définis et des banques de peptides, afin d'augmenter les niveaux d'expression cellulaire, réduire le catabolisme cellulaire, accroître la stabilité conformationnelle des peptides linéaires et augmenter les concentrations d'état stationnaire des peptides aléatoires et des éléments de banques de peptides aléatoires exprimés dans des cellules, de manière à détecter la présence de peptides et à cribler les banques de peptides aléatoires. L'invention porte également sur le N-terminal, le C-terminal, la combinaison de ceux-ci et sur une ou plusieurs fusions internes. Sont également abordées de nouvelles fusions faisant intervenir des peptides auto-liants en vue de la création d'une fusion à conformation stabilisée.

Affiliated with

Also associated with

Rating

Fusions of scaffold proteins with random peptide libraries does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fusions of scaffold proteins with random peptide libraries, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fusions of scaffold proteins with random peptide libraries will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1414392