C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/29 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01) G01N 33/566 (2006.01)
Patent
CA 2258694
A method of altering G protein signal transduction pathways in plants and thereby influencing plant growth, development and responses to endogenous signalling molecules, environmental signals, pests and pathogens is disclosed herein. A G protein-coupled receptor in Arabidopsis thaliana, Brassica napus and Brassica oleracea have been discovered, isolated and cloned. Specifically, the cDNA sequence encoding the full length receptor Arabidopsis thaliana has been isolated and purified, as well as the amino acid sequence. The isolated G protein-coupled receptor of the present invention is believed to influence the sensitivity of plants to cytokinins.
Procédé de modification des voies de transduction de signal de protéine G dans des plantes, ce qui permet d'influer sur la croissance de la plante, son développement et ses réactions à des molécules endogènes de signalisation, à des signaux émis par le milieu, à des parasites et à des agents pathogènes. On a découvert, isolé et cloné un récepteur couplé à la protéine G dans Arabidopsis thaliana, Brassica napus et Brassica oleracea. On a spécifiquement isolé et purifié la séquence d'ADNc codant la longueur totale du récepteur d'Arabidopsis thaliana, ainsi que sa séquence d'acides aminés. On pense que ce récepteur isolé couplé à la protéine G exerce une influence sur la sensibilité des plantes aux cytokinines.
Hooley Richard
Plakidou-Dymock Stella
Sim & Mcburney
University Of Bristol
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