Ga4 dna, protein and methods of use

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2197753

The invention relates to the DNA and protein encoded by the GA4 locus. This protein is believed to be a member of the family of enzymes involved in the biosynthesis of the gibberellin family (GA) of plan growth hormones which promote various growth and developmental processes in higher plants, such as seed germination, stem elongation, flowering and fruiting. More specifically, the protein encoded by the GA4 locus is an hydroxylase. The invention also relates to vectors containing the DNA and the expression of the protein encoded by the DNA of the invention in a host cell. Additional aspects of the invention are drawn to host cells transformed with the DNA or antisense sequence of the invention, the use of such host cells for the maintenance, or expression or inhibition of expression of the DNA of the invention and to transgenic plants containing DNA of the invention. Finally, the invention also relates to the use of the protein encoded by the GA4 locus to alter aspects of plant growth.

ADN et protéine codée par le locus de GA4. Cette protéine serait un membre de la famille des enzymes impliqués dans la biosynthèse de la famille gibbérelline (GA) des hormones de croissance des végétaux qui favorisent divers processus de croissance et de développement chez les plantes supérieures, comme la germination des graines, la croissance de la tige, la floraison et la fructification. De manière plus spécifique, la protéine codée par le locus de GA4 est une hydroxylase. L'invention porte également sur des vecteurs renfermant l'ADN et l'expression de la protéine codée par l'ADN visé dans cette invention dans une cellule hôte. Elle porte également sur d'autres aspects tels que des cellules hôtes transformées par l'ADN ou la séquence antisens visée ici, l'utilisation de telles cellules hôtes pour la préservation, l'expression ou l'inhibition de l'expression de cet ADN, et sur des végétaux transgéniques renfermant cet ADN. Enfin, elle porte également sur l'utilisation de la protéine codée par le locus de GA4 pour modifier certains aspects de la croissance des végétaux.

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