Galectin-3 immunoassay

G - Physics – 01 – N

Patent

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Details

G01N 33/68 (2006.01)

Patent

CA 2742265

The present invention relates to methods and compositions for specifically and quantitatively detecting galectin-3 in a sample. Embodiments of the invention include a detection assay in which a capture binding moiety and a labeled binding moiety specifically recognize non-overlapping epitopes on the N-terminus of galectin-3. Further embodiments are directed to a method for establishing ranges of galectin-3 concentrations indicative of the presence and severity of heart failure in a subject and a method for predicting the clinical outcome of a subject based upon galectin- 3 concentration.

La présente invention concerne des procédés et des compositions pour détecter spécifiquement et quantitativement la galectine-3 dans un échantillon. Des modes de réalisation de l'invention comprennent un essai de détection dans lequel un fragment de liaison de capture et un fragment de liaison marqué reconnaissent spécifiquement des épitopes non chevauchants sur la terminaison N de galectine-3. D'autres modes de réalisation concernent un procédé pour établir des plages de concentrations de galectine-3 indicatrices de la présence et de la gravité d'une insuffisance cardiaque chez un sujet et un procédé pour prédire le résultat clinique d'un sujet sur la base de la concentration de galectine-3.

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