Gel cassette for enhanced electrophoretic separation and...

G - Physics – 01 – N

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G01N 27/447 (2006.01)

Patent

CA 2170353

A gel cassette useful in electrophoretic separation of molecular fragments is dis- closed, in which a gelatinous substrate is uniquely configured to concentrate similar separated fragments. Containment means is also disclosed which provides for the desired configuration of the substrate. The contain- ment means further includes a cover design which locks into an open position to elimi- nate cross contamination. In addition, pro- cesses for the preparation of such cassettes and for use of the cassettes in separation and transfer apparatus are disclosed.

L'invention se rapporte à une cassette de gel apte à être utilisée pour la séparation électrophorétique de fragments moléculaires, dans laquelle un substrat gélatineux est doté d'une configuration unique lui permettant de concentrer des fragments séparés semblables. L'invention se rapporte également à des moyens de confinement permettant de conférer audit substrat la configuration requise. Le moyen de confinement comprend en outre un modèle de couvercle qui se verrouille en une position ouverte afin de supprimer les risques de contamination croisée. Par ailleurs, des procédés de préparation de telles cassettes et d'utilisation de ces dernières dans des dispositifs de séparation et de transfert sont décrits.

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