Gel microdrops in genetic analysis

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2339734

The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.

L'invention concerne des méthodes d'analyse d'acides nucléiques. Ces méthodes consistent à former une population de microgouttelettes de gel encapsulant une population d'entités biologiques où chacune des entités contient un acide nucléique. Dans un nombre minimum de ces microgouttelettes chaque microgouttelette encapsule une seule entité. On met ensuite la population de microgouttelettes de gel en contact avec une sonde dans des conditions où la sonde s'hybride avec au moins une séquence supplémentaire de l'acide nucléique dans au moins une microgouttelette de gel. On analyse ou on détecte ensuite au moins une microgouttelette de gel. Les entités biologiques peuvent être des cellules, des virus, des noyaux ou des chromosomes.

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