Gene coding for the e1 endoglucanase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) C07K 19/00 (2006.01) C12N 9/42 (2006.01) C12N 15/62 (2006.01) C12P 7/10 (2006.01) C12P 19/14 (2006.01)

Patent

CA 2194478

The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in heterologous microorganisms. A new modified E1 endoglucanase enzyme is produced along with variants of the gene and enzyme. The E1 endoglucanase is useful for hydrolyzing cellulose to sugars for simultaneous or later fermentation into alcohol.

Le gène codant l'endoglucanase E1 d'Acidothermus cellulolyticus est cloné et exprimé dans des micro-organismes hétérologues. Une nouvelle endoglucanase E1 modifiée est produite avec des allèles de ce gène et de cette enzyme. Ladite endoglucanase E1 est utile pour hydrolyser la cellulose en sucres en vue de sa fermentation simultanée ou ultérieure en alcool.

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