Gene encoding cyclic lipopeptide acylase and expression of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/55 (2006.01) C12N 1/21 (2006.01) C12N 9/14 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2377793

Determination of the base sequence in the coding domain of a cyclic lipopetide acylase; determination of the full amino acid sequence of this acylase; an expression vector containing a gene encoding the above enzyme; and a process for producing the cyclic lipopeptide acylase by expressing the above expression vector in a host cell. Use of a transformant, which has an activity comparable to acylase obtained by culturing a conventional cyclic lipopeptide acylase-producing strain and has been constructed genetic engineeringly, makes it possible to shorten the culture time (i.e., the time for producing acylase).

L'invention se rapporte à la détermination de la séquence de base du domaine de codage d'une acylase des lipopeptides cycliques; à la détermination de la totalité de la séquence d'acides aminés de cette acylase; à un vecteur d'expression contenant un gène codant pour l'enzyme ci-dessus; et à un procédé de production de cette acylase des lipopeptides cycliques par expression du vecteur d'expression ci-dessus dans une cellule hôte. L'invention se rapporte également à un transformant, qui possède une activité comparable à l'acylase que l'on obtient en cultivant une souche classique productrice d'acylases des lipopeptides cycliques et a été manipulé génétiquement, et dont l'utilisation permet de réduire le temps de culture (c'est à dire le temps nécessaire à la production de cette acylase).

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