Gene encoding recombinant trehalose phosphorylase, vector...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/14 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12P 19/18 (2006.01)

Patent

CA 2285232

A novel recombinant trehalose phosphorylase. A gene encoding trehalose phosphorylase is found out and a vector containing the same is constructed. Then a transformant is prepared and a recombinant trehalose phosphorylase is produced with the use of this transformant. Thus, trehalose phosphorylase can be produced on an industrial scale and, by using this enzyme, trehalose can be industrially advantageously produced from D-glucose and .alpha.-D-glucose-1- phosphate.

L'invention concerne une nouvelle tréhalose phosphorylase recombinée. L'invention concerne également la découverte d'un gène codant ladite tréhalose phosphorylase recombinée, ainsi que la construction d'un vecteur renfermant ce gène. L'invention concerne en outre un procédé de préparation d'un transformant ainsi qu'un procédé de production d'une tréhalose phosphorylase recombinée, au moyen de ce transformant. Cette invention permet donc de produire la tréhalose phosphorylase à l'échelle industrielle, et, au moyen de cette enzyme, de produire industriellement le tréhalose à partir de D-glucose et de .alpha.-D-glucose-1-phosphate.

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