Gene expression assays conducted by elemental analysis

Details Gene expression assays conducted by elemental analysis Gene expression assays conducted by elemental analysis

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01) C12Q 1/00 (2006.01) C40B 30/04 (2006.01) C40B 70/00 (2006.01) G01N 33/483 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2640508

Methods and kits for gene expression analysis are disclosed. The methods utilize element-tagged oligonucleotides as probes which are subsequently analyzed by elemental analysis.Also disclosed are methods and kits for the analysis of biological molecules using element labeled supports such as beads, followed by elemental analysis. The elemental analysis can be done using inductively coupled plasma mass spectrometry (ICP-MS).

L'invention concerne des procédés et des trousses pour analyse de l'expression génique. Ces procédés font intervenir des ologonucléotides marqués par élément qui sont soumis ultérieurement à une analyse élémentaire, L'invention concerne également des procédés et des trousses utilisés pour l'analyse de molécules biologiques au moyen de supports marqués par élément tels que des perles, suivie d'une analyse élémentaire. L'analyse élémentaire peut être effectuée par spectrométrie de masse à émission de plasma à couplage inductif (ICP-MS).

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